Department of Biology Technion Israel Institute of Technology Haifa 32000 Israel

  1. Introduction to Proteomics 160
  2. Modern Proteomic Technology 160
  3. 1. 2D-PAGE 161
  4. 2. HPLC 162
  5. 3. Mass Spectrometry 163
  6. 4. Protein Arrays 165
  7. 5. Bioinformatics 165
  8. 6. Technology Portability, Reproducibility, and

Performance Characteristics 166

  1. Proteins, Proteomics, and Patterns 167
  2. Accessing Biological Samples 169
  3. 1. Invasion Interface 170
  4. 2. Laser Capture Microdissection 171
  5. Preparing and Resolving Complex Specimens 172
  6. 1. Optimizing Biological Samples 172
  7. 2. Subfractionation of Protein Mixtures 173
  8. Standardization of Proteomic Approaches 174
  9. Combining Modern Proteomic Technologies with

Traditional Histopathology Techniques 175

  1. Practical Clinical Applications 176
  2. 1. Tumor Markers 177
  3. 2. Disease-Related Applications 178
  4. Future Directions 179

References 180

Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), high-performance liquid chromatography (HPLC), mass spectrometry (MS), tandem mass spectrometry (MS/MS), post-translational modifications (PTMs), time of flight (TOF), multi-dimensional protein identification technology (MudPIT), enzyme-linked immunosorbent assay (ELISA), cancer antigen 125 (CA125), laser capture microdissection (LCM), cholamidoprophy dimethalammonio-1-propanesufonate (CHAPS), polymerase chain reaction (PCR), Human Proteome Organization (HUPO), Proteomics Standards Initiative (PSI), hematoxylin and eosin (H&E), Terry's Polychrome, (TP), Toluidine Blue (TB), Nuclear Fast Red (NFR), Cresyl Violet (CV), Methylene Blue (MB), tumor-specific antigens (TSAs), tumor-associated antigen (TAA), hepatocellular carcinoma (HCC), prostate specific antigen (PSA), graft-versus-host disease (GVHD), CD8 anti-viral factor (CAF), human immunodeficiency virus (HIV).

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