Department of Biology Technion Israel Institute of Technology Haifa 32000 Israel
- Introduction to Proteomics 160
- Modern Proteomic Technology 160
- 1. 2D-PAGE 161
- 2. HPLC 162
- 3. Mass Spectrometry 163
- 4. Protein Arrays 165
- 5. Bioinformatics 165
- 6. Technology Portability, Reproducibility, and
Performance Characteristics 166
- Proteins, Proteomics, and Patterns 167
- Accessing Biological Samples 169
- 1. Invasion Interface 170
- 2. Laser Capture Microdissection 171
- Preparing and Resolving Complex Specimens 172
- 1. Optimizing Biological Samples 172
- 2. Subfractionation of Protein Mixtures 173
- Standardization of Proteomic Approaches 174
- Combining Modern Proteomic Technologies with
Traditional Histopathology Techniques 175
- Practical Clinical Applications 176
- 1. Tumor Markers 177
- 2. Disease-Related Applications 178
- Future Directions 179
References 180
Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), high-performance liquid chromatography (HPLC), mass spectrometry (MS), tandem mass spectrometry (MS/MS), post-translational modifications (PTMs), time of flight (TOF), multi-dimensional protein identification technology (MudPIT), enzyme-linked immunosorbent assay (ELISA), cancer antigen 125 (CA125), laser capture microdissection (LCM), cholamidoprophy dimethalammonio-1-propanesufonate (CHAPS), polymerase chain reaction (PCR), Human Proteome Organization (HUPO), Proteomics Standards Initiative (PSI), hematoxylin and eosin (H&E), Terry's Polychrome, (TP), Toluidine Blue (TB), Nuclear Fast Red (NFR), Cresyl Violet (CV), Methylene Blue (MB), tumor-specific antigens (TSAs), tumor-associated antigen (TAA), hepatocellular carcinoma (HCC), prostate specific antigen (PSA), graft-versus-host disease (GVHD), CD8 anti-viral factor (CAF), human immunodeficiency virus (HIV).
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