Fluorescent Agents

Confocal endomicroscopy uses stimulated light emission and relies on the application of fluorescent agents. Fluo-rescein, acriflavine, t etracycline and, to a lesser extent, cresyl violet are compatible with currently available laser light and filtering options and have been studied in animals and human subjects. In most studies, intravenous fluorescein sodium or topical acriflavine hydrochloride were evaluated (O Fig. 4.7).

of the mucin inclusion (arrows). Right panel: After fluorescein injection, the cytoplasm of the cells is highlighted, while the nuclei cannot be directly visualised. Mucin inside the goblet cells remains black (arrows). Capillaries are not seen at superficial sections of the epithelial layer

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