Additional reading for Part III

Note: see Resource Center for relevant websites

Alberts BA, Johnson A, Lewis J, Raff M, Roberts K, Walter P. Molecular biology of the cell, 4th ed. New York: Garland, 2002. Basic genetic mechanisms: DNA replication (Chapter 5:238266). Basic genetic mechanisms: RNA and protein synthesis (Chapter 6:300-372). Recombinant DNA technology: the fragmentation, separation, and sequencing of DNA molecules (Chapter 8:491-513). Recombinant DNA technology: nucleic acid hybridization (Chapter 8:495-500).

Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K, eds. Current protocols in molecular biology. New York: John Wiley and Sons. 1994-99. Volume 1: Section 1. Escherichia coli, plasmids, and bacteriophages: Part II. Vectors derived from plasmids. Section 2. Preparation and analysis of DNA: Part IV. Analysis of DNA sequences by blotting and hybridization. Section 3. Enzymatic manipulation of DNA and RNA: Part I. Restriction endonucleases. Part II. Enzymatic manipulation of DNA and RNA: restriction mapping. Section 4. Preparation and analysis of RNA: Part IV. Analysis of RNA structure and synthesis. Section 7. DNA sequencing: Part I. DNA sequencing strategies. Section 9. Introduction of DNA into mammalian cells: Part I. Transfection of DNA into eukaryotic cells. Volume 2: Section 10. Analysis of proteins: Part III. Detection of proteins. Sub-section 10.7. Detection of proteins on blot transfer membranes. Sub-section 10.8. Immunoblotting and immunodetection. Section 11. Immunology: Part I. Immu-noassays: Subsection 11.2. Enzyme-linked immunosorbent assay (ELISA). Section 14. In situ hybridization and immu-nohistochemistry: Subsection 14.3. In situ hybridization to cellular RNA. Section 15. The polymerase chain reaction: Subsection 15.1. Enzymatic amplification of DNA by the polymerase chain reaction: standard procedures and optimization.

Celis A, Celis JE. General procedures for tissue culture. In Celis J, ed. Cell biology: a laboratory handbook, 2nd ed, vol 1. San Diego: Academic Press, 1998:5-15.

Celis A, Dejgaard K, Celis JE. Production of mouse monoclonal antibodies. In Celis J, ed. Cell biology: a laboratory handbook, 2nd ed, vol 2. San Diego: Academic Press, 1998:392-397.

Celis JE, Olsen E. One-dimensional sodium dodecyl sulfate— Polyacrylamide gel electrophoresis. In Celis J, ed. Cell biology: a laboratory handbook, 2nd ed, vol 4. San Diego: Academic Press, 1998:361—370.

Coen DM, Ramig RF. Viral genetics. In Fields BN, Knipe DM, eds. Virology, 3rd ed. New York: Raven Press, 1995: Chapter 5.

Cristofalo VJ, Charpentier R, Philips PD. Serial propagation of human fibroblasts for the study of aging at the cellular level. In Celis J, ed. Cell biology: a laboratory handbook, 2nd ed, vol 1. San Diego: Academic Press, 1998:321—326.

Davis RH, Weller SG. The mutational process. In The gist of genetics. Boston: Jones and Bartlett, 1997: Chapter 16.

Hartl DL, Jones EW. Mutation, DNA repair, and recombination. In Essential genetics, 3rd ed. Boston: Jones and Bartlett, 2002: Chapters 4 and 7.

Janeway C, Travers P, Hunt S, Walport M. Immunobiology, 6th ed. New York: Garland, 2004: Chapter 2.

Kroes A, Kox L. Detection of viral antigens, nucleic acids and specific antibodies. In Webster RG, Granoff A, eds. Encyclopedia of virology, 2nd ed. New York: Academic Press, 1999.

Landry ML, Hsiung G-D. Isolation and identification by culture and microscopy. In Webster RG, Granoff A, eds. Encyclopedia of virology, 2nd ed. New York: Academic Press, 1999.

Leland DS. Concepts of immunoserological and molecular techniques. In Clinical virology. Philadelphia: WB Saunders, 1997: Chapter 2.

Leland DS. Virus isolation in traditional cell cultures. In: Clinical virology. Philadelphia: WB Saunders, 1997: Chapter 3.

Lewin B. Genes VIII. New York: Oxford Press, 2003. Isolating the gene: a restriction map is constructed by cleaving DNA into specific fragments. Restriction sites can be used as genetic markers. Obtaining the sequence of DNA (Chapter 6). Messenger RNA (Chapter 7). Protein synthesis (Chapter 8).

Lodish H, Baltimore D, Berk A, Zipursky SL, Matsudaira P, Darnell JE. Transcription termination, RNA processing, and post-transcriptional control: mRNA processing in higher eucary-otes. In Molecular cell biology, 4th ed. New York: Scientific American, 2000: Chapter 12.

Maunsbach AB. Fixation of cells and tissues for transmission electron microscopy. In Celis J, ed. Cell biology: a laboratory handbook, 2nd ed, vol 2. San Diego: Academic Press, 1998:249259.

Osborn M. Immunofluorescence microscopy of cultured cells. In Celis J, ed. Cell biology: a laboratory handbook, 2nd ed, vol 2. San Diego: Academic Press, 1998:462-468.

Pawley JB, Centonze VE. Practical laser-scanning confocal light microscopy: obtaining optimal performance from your instrument. In Celis J, ed. Cell biology: a laboratory handbook, 2nd ed, vol 3. San Diego: Academic Press, 1998:149169.

Whitaker-Dowling P, Youngner JS. Virus-host cell interactions. In Webster RG, Granoff A, eds. Encyclopedia of virology, 2nd ed. New York: Academic Press, 1999.

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