Chapter

  • VIRAL STRUCTURAL PROTEINS
  • Isolation of structural proteins of the virus
  • Size fractionation of viral structural proteins Determining the stoichiometry of capsid proteins

The poliovirus capsid - a virion with equimolar capsid proteins Analysis of viral capsids that do not contain equimolar numbers of proteins

  • CHARACTERIZING VIRAL GENOMES
  • Sequence analysis of viral genomes
  • Measuring the size of viral genomes

Direct measure of DNA genome lengths in the electron microscope Rate zonal sedimentation and gel electrophoresis for measuring viral genome size

# The polymerase chain reaction - detection and characterization of extremely small quantities of viral genomes or transcripts

Real time PCR for precise quantitative measures of viral DNA

PCR detection of RNA

PCR as an epidemiological tool

# QUESTIONS FOR CHAPTER 11

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