Measuring the size of viral genomes

While nucleotide sequence of a viral genome defines the genome's molecular size, a number of very accurate physical and biochemical methods for measuring genome size were developed well in advance of sequencing. These methods are still in occasional use and many important publications refer to them; therefore, a brief description of a few that were most widely used is valuable.

Gel

T

Pr

imer-TGCGTCCACAACGCT

C

Pr

mer-TGCGTCCACAACGC

G

Pr

mer-TGCGTCCACAACG

C

Pr

mer-TGCGTCCACAAC

A

Pr

mer-TGCGTCCACAA

A

Pr

imer-TGCGTCCACA

C

Pr

imer-TGCGTCCAC

A

Pr

imer-TGCGTCCA

C

Pr

imer-TGCGTCC

C

Pr

imer-TGCGTC

T

Pr

imer-TGCGT

G

Pr

imer-TGCG

C

Pr

imer-TGC

G

Pr

imer-TG

T

Pr

TGCGT CC ACAACGCT

Fluorescent signal intensity and wavelength

Fig. 11.8 Automated DNA sequencing. In a typical sequencing reaction, each of the dideoxynucleotides is labeled with a specific dye that fluoresces to emit a given wavelength of light. Sensors measure the wavelength as the overlapping fragments of DNA in the reaction mixture are separated by electrophoresis and move past the detecting site. The results are recorded and stored in a database for later sequence intepretation.

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