Questions For Chapter

1 You have encountered a virus named hotvirus with that were uniformly radiolabeled with radioactive amino three capsid proteins, E, K, and W. After gel acids, you obtain the following results:

fractionation of a purified stock of pure viral capsids

Protein

Molecular weight

Radioactivity (cpm)

E

5,280

29,348

K

18,795

101,185

W

10,776

122,674

What are the best values for the ratios of the proteins E to K to W?

What are the best values for the ratios of the proteins E to K to W?

  • a) Which of these isolates is potentially a virus identical to or very closely related to poliovirus type 1?
  • b) Which of these isolates may be another member of the same family as poliovirus type 1?
  • c) From which family of viruses might isolate B come? (Note: you will probably have to do some searching in Chapter 14 to find properties of enteric viruses in order to answer this question.)

3 How is SDS polyacrylamide gel electrophoresis used for the analysis of proteins? What is the basis for this technique?

4 Besides the molar ratio of the proteins, what would you need to know to determine the amount of specific proteins per capsid in a particular virus?

5 While analyzing the structural proteins of a pure stock of adenovirus by SDS polyacrylamide gel electrophoresis, you find, among others, two bands of equal intensity that migrate at 30,000 and 60,000 daltons, respectively. What conclusion can you draw from this observation?

2 Your laboratory has isolated a number of possible enteric viruses from samples of contaminated water. You have grown these viruses in appropriate cell cultures and have labeled the proteins with 35S-methionine. You have purified virus particles from these cultures and separated the capsid proteins by sodium dodecyl sulfate (SDS) polyacrylamide gel electrophoresis. Below is an autoradiogram of this experiment with poliovirus type 1 (PV1) included as a control:

Isolate

PV1

A

B

C

»

VP1

Poliovirus

VP2

capsid

VP3

proteins

_ VP4

«

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