K

Leukocytes Earthworm

A Protein elusion profile of ' m a superdex 200column. (230 nm) C Western blotting of fraction 5 to 11 Fig. 3 Protein purification, SDS-PAGE and immunoblotting. (A) Protein elution profiles of coelomocyte lysates run on a SuperDex 200 column (B) SDS-PAGE of a number of coelomocyte lysate fractions (C) By western blot three protein bands were identified with the anti-perforin antibody. Molecular mass of these proteins was determined as of about 68, 70 and 72 kDa. ionomycin. However, cytotoxic...

Info

Cell adhesion activity detected from purified protein. 1 cell adhesion activity detected in hemolymph 2 cell adhesion activity detected in recombinant protein expressed in baculovirus vector cell adhesion activity detected from purified protein. 1 cell adhesion activity detected in hemolymph 2 cell adhesion activity detected in recombinant protein expressed in baculovirus vector To avoid deleterious effects, the proPO system has to be controlled and regulated. Several inhibitors of the proPO...

Makltlassp Wflsvswrsl Ppllssrnal Mmdgrtdgln Qsgrkrtsll Gsgatlleig Lvmltikvsr Parttdstps Qlsslnsatr Tktlvcqfsv

Molecular mass 1107.49 SEVSEETAEK Molecular mass 1146.56 KPDGYDDIPK Molecular mass 1221.59 DDPELYVFPK This work supported by the Foundation for Scientific Research-Flanders (G.0169.01), the Grant Agency of the Academy of Sciences of the Czech Republic (A5020208), and by Institutional Research Concept (AV0Z5020903) was performed in frame of an Interuniversity Attraction Pole Program. 1 P. Roch, Protein analysis of earthworm coelomic fluid. I. Polymorphic system of the natural hemolysins of...

New Model For Analyzing Antimicrobial Peptides With Biomedical Applications

A series presenting the results of scientific meetings supported under the NATO Science Programme. The series is published by IOS Press and Kluwer Academic Publishers in conjunction with the NATO The NATO Science Series continues the series of books published formerly as the NATO ASI Series. The NATO Science Programme offers support for collaboration in civil science between scientists of countries of the Euro-Atlantic Partnership Council. The types of scientific meeting generally supported are...

Leech immune responses contributions and biomedical applications

Magda de EGUILEOR, Gianluca TETTAMANTI, Annalisa GRIMALDI, Roberto FERRARESE, Gianpaolo PERLETTI, Roberto VALVASSORI, Edwin L.COOPER Giulio LANZAVECCHIA Department of Structural and Functional Biology, University of Insubria, Via J.H.Dunant 3 21100 Varese, Italy 1 Laboratory of Comparative Immunology, Department of Neurobiology School of Medicine, University of California, Los Angeles, CA 90095-1763, USA Abstract. Leeches can use a variety of selected defense systems against nonself. Different...

Pbs Hbss L15

Dehydrogenase Activity With Temperature

Fig. 4 In vitro viability of Eisenia foetida coelomocytes after 7 h incubation in PBS, HBSS or L-15 means SD. Different letters indicate mean values significantly different. 20 100 220 285 320 400 600 osmolarity mOsm Fig. 5 In vitro viability of Eisenia foetida coelomocytes after 2 h incubation in PBS of various osmolalities means SD. Different letters indicate mean values significantly different. 20 100 220 285 320 400 600 osmolarity mOsm Fig. 5 In vitro viability of Eisenia foetida...

M

Lysenin Oligomerize Electron

Fig. 1 Eiseniapore induced leakage of small unilamilar vesicles (SUVs) of different lipid composition. A. Eiseniapore induced leakage of SUVs at pH 7.4 and 25 C for vesicles of different lipid composition (PC- phosphatidylcholine, CHOL- cholesterol, PE-phosphatidylethanolamine and PS- phosphatidylserine). B. Eiseniapore induced leakage of liposomes with different sphingolipid composition. Leakage was measured after 5 min incubation with eiseniapore. Values above the component indicate leakage...

An overview of cytolytic factors in Eisenia foetida coelomic fluid

Martin BILEJ, Ellen KAUSCHKE Kazuo KOMIYAMA 2, Philippe ROCH Petra KOHLEROVA, Marcela SILEROVA, Edwin L. COOPER 4, Patrick DE BAETSELIER 5, Ludmila TUCKOVA, Alain BESCHIN 5 Department of Immunology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic 'institute of Anatomy, Ernst-Moritz-Arndt-University Greifswald, Friedrich-Loeffler-Strafie 23c, D-l 7489 Greifswald, Germany 2 Department of Pathology, Nihon University School of...

Printed In The Netherlands

Director of the Institute of Microbiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic It is a great pleasure to welcome the participants to this NATO Advanced Research Workshop. As one of the co-sponsors, we are pleased since the subject of the workshop fits most appropriately within the context of certain ongoing projects. In this context, it is instructive to offer some history and overview of our activities. The Institute of Microbiology, Czechoslovak Academy of...

Evidence for a perforinlike cellmediated cytolysis by earthworm coelomocytes

Kazuo KOMIYAMA, Ellen KAUSCHKE Edwin L. COOPER2 Department of Pathology, Nihon University School of Dentistry, 1-8-13 Kanda-Surugadai, Chiyoda-ku, Tokyo 1018310, Japan 1 Institute of Anatomy, Ernst-Moritz-Arndt-University Greifswald, Friedrich-Loejfler-Strafie 23c, D-l 7489 Greifswald, Germany 2 Laboratory of Comparative Immunology, Department of Neurobiology, School of Medicine, University of California, Los Angeles, CA 90095-1763, USA Abstract. Small earthworm leukocytes express lytic...

Coelomic fluid from Lumbricus terrestris and Eiseniafoetida inhibits Escherichia coli

Milton Academy, 170 Centre Street Milton, Massachusetts 02186 Abstract. This study shows preliminary evidence of antimicrobial molecules and their dose-dependent activities in earthworm coelomic fluid. Coelomic fluids from Lumbricus terrestris and Eisenia foetida, when compared to controls 1) suppressed bacterial growth of Esherichia coli K-12, as measured in colony forming units (cfu) 2) increased the area inhibition zones as measured in centimeter square. Antimicrobial activities from both...

Comparative analysis of earthworm immune system using cell surface and intracellular markers

COOPER P ter NEMETH Department of Immunology and Biotechnology, School of Medicine, University of P cs, P cs, Hungary 'Laboratory of Comparative Immunology, Department of Neurobiology, School of Medicine, University of California, Los Angeles, CA 90095-1763, USA Abstract. Coelomocytes located in the coelomic cavity of the earthworms Eisenia foetida are involved in the immune protection of these animals. Three coelomocyte subpopulations were identified by flow...

The prophenoloxidase activating system in invertebrates a short review

Kenneth SODERHALL and Kallaya SRITUNYALUCKSANA Department of Comparative Physiology, Evolutionary Biology Centre, Uppsala University, Norbyvagen 18A, Uppsala, S-752 36, Sweden Abstract. Invertebrate animals do not have antibodies and therefore have to rely on innate immune systems, but still they have to be able to recognize foreign materials and respond to it so that appropriate measures are initiated to combat and destroy invading microorganisms. The ways in which invertebrate animals...

Mitogenic factors in Eisenia foetida coelomic fluid as regulatory molecules

Martin BELEJ, RadkaHANUSOVA, Petra KOHLEROVA, Patrick DE BAETSELEER Department of Immunology, Institute of Microbiology, Academy of Sciences of the Czech Republic, Videnska 1083, 142 20 Prague 4, Czech Republic 1 Department of Immunology, Parasitology and infrastructure, Flemish Interuniversity Institute for Biotechnology, Free University Brussels (VUB), Paardenstraat 65, B-1640 St-Genesius-Rode, Belgium Abstract. Free coelomocytes that are derived from mesenchymal lining of the coelomic cavity...

New powerful cascade coagulant inhibitors isolated from the gut of the leech Theromyzon tessulatum

Pierre-Eric SAUTI RE, Aurelie TASIEMSKI, Franck VANDENBULCKE, Annie DESMON, Michel SALZET Laboratoire de Neuroimmunologie des Ann lides, ESA CNRS 8017, SN3,IFR 17INSERM, Universit des Sciences et Technologies de Lille, 59655 Villeneuve d'Ascq C dex, France Abstract. Thrombin and factor Xa inhibitors, have already been isolated from jawed and gut leeches respectively. In this review, we report for the first time the isolation of both serine protease inhibitors in the same species, the gut leech...