Info

Control 24 Hr 24 Hr 24 Hr 24 Hr 24 Hr 48 Hr 48 Hr 48 Hr 48 Hr 48 Hr

base DCC FRS base DCC FRS

Preservation solution

Control 24 Hr 24 Hr 24 Hr 24 Hr 24 Hr 48 Hr 48 Hr 48 Hr 48 Hr 48 Hr

base DCC FRS base DCC FRS

Preservation solution

FIGURE 6.9 A comparison between viability and functional activity in neonatal rat ventricular cardiomyo-cytes (NRVM). NRVM were isolated through proteolytic digestion, plated in 24 well plates, and maintained at 37oC for 2 days. The cultures were then immersed for 24 hours at 4oC in a variety of hypothermic solutions that included standard culture media, ViaSpan ("UW"), HTS-BASE, HTS-DCC, and HTS-FRS. Cultures were scored first for the percent cells contracting (tubes, foreground) and subsequently for membrane integrity (bars, background) using the Calcein-AM test. Note that there was a correlation between the viability and functional asssays.

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