Signal Mediated Amplification of RNA Technology

Signal-mediated amplification of RNA technology (SMART) is a novel isothermal amplification technology that uses a three-way junction (3WJ) structure to facilitate target-dependent production of multiple copies of a RNA product (Wharam et al., 2001). The 3WJ structure is composed of two target-specific single-stranded DNA probes (the template probe and the extension probe) and a target sequence. Both probes have a longer region that hybridizes to the target at adjacent sites and a shorter...