Contents

Part I Techniques

1 Automated Blood Cultures 3

Xiang Y. Han

2 Urea Breath Tests for Detection of Helicobacter pylori 11

Sihe Wang and Xiaotian Zheng

3 Rapid Antigen Tests 23

Sheldon Campbell and Marie L. Landry

4 Advanced Antibody Detection 42

5 Phenotypic Testing of Bacterial Antimicrobial Susceptibility 63

Chao Qi, Charles W. Stratton, and Xiaotian Zheng

6 Biochemical Profile-Based Microbial Identification Systems 84

Jaber Aslanzadeh

7 Rapid Bacterial Characterization and Identification by

MALDI-TOF Mass Spectrometry 117

Diane Dare

8 Probe-Based Microbial Detection and Identification 134

Tao Hong

9 Pulsed-Field Gel Electrophoresis 143

Fann Wu and Phyllis Della-Latta

10 In Vitro Nucleic Acid Amplification: An Introduction 158

Haijing Li and Yi-Wei Tang

11 PCR and Its Variations 166

Michael Loeffelholz and Helen Deng

12 Non-Polymerase Chain Reaction Mediated Target Amplification Techniques 184

Michael L. Pendrak and S. Steve Yan

13 Recent Advances in Probe Amplification Technologies 210

David Zhang, Tao Feng, Fei Ye, Ivy Lee, Josephine Wu, and Bingjiao Yin

14 Signal Amplification Techniques: bDNA, Hybrid Capture 228

15 Detection and Characterization of Molecular Amplification Products: Agarose Gel Electrophoresis, Southern Blot Hybridization, Restriction Enzyme Digest Analysis, and Enzyme-Linked Immunoassay 243

Raymond P. Podzorski, Mike Loeffelholz, and Randall T. Hayden

16 Direct Nucleotide Sequencing for Amplification Product Identification 264

Tao Hong

17 Microarray-Based Microbial Identification and Characterization 276

Terry J. Gentry and Jizhong Zhou

18 Diagnostic Microbiology Using Real-Time PCR Based on FRET

Technology 291

Xuan Qin

19 Amplification Product Inactivation 306

Susan Sefers and Yi-Wei Tang

Part II Applications

20 Bacterial Identification Based on 16S Ribosomal RNA Gene

Sequence Analysis 323

Xiang Y. Han

21 Molecular Techniques for Blood and Blood Product Screening 333

Yuan Hu and Irvin Hirshfield

22 Review of Molecular Techniques for Sexually Transmitted

Diseases Diagnosis 353

Angus C.T. Lo and Kai Man Kam

23 Advances in the Diagnosis of Mycobacterium tuberculosis and

Detection of Drug Resistance 387

Abdullah Kilic and Wonder Drake

24 Rapid Screening and Identification of Methicillin-Resistant

Staphylococcus aureus 411

Patrice Francois and Jacques Schrenzel

25 Bead-Based Flow Cytometric Assays: A Multiplex Assay Platform with Applications in Diagnostic Microbiology 427

David Ernst, George Bolton, Diether Recktenwald, Mark J. Cameron, Ali Danesh, Desmond Persad, David J. Kelvin, and Amitabh Gaur

26 Molecular Strain Typing Using Repetitive Sequence-Based PCR 444

Stacie R. Frye and Mimi Healy

27 Molecular Differential Diagnoses of Infectious Diseases: Is the

Future Now? 472

Jian Han

28 Pathogen Detection in the Genomic Era 505

Elizabeth M. Marlowe and Donna M. Wolk

Index 525

Parti

Techniques

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