Specimen Automation Nucleic Acid Extraction and Molecular Diagnostics

Efficient extraction and preparation of a specimen for nucleic acid analysis is critical to the quality of molecular testing results. During the past decade, improvements in novel and convenient extraction schemes have allowed the routine integration of molecular testing in many clinical diagnostic laboratories. More recently, the evolution of extraction systems into an automated format allows for the common practice of molecular testing. Several reviews serve as a reference for specific details of extraction chemistries and real-time instruments (Wolk et al., 2001; Wolk and Persing, 2002).

Specimen preparation automation can be divided into three categories: (i) nucleic acid extraction, (ii) specimen processing/PCR assay set-up, and (iii) general liquid handing systems. Some systems combine part or all of these categories into one system (Table 28.1). Many companies developed these automated systems so they can be integrated with their real-time PCR instruments for complete automation of extraction, sample processing, and amplification/detection.

Table 28.1. Automated specimen processing and real-time instruments.

Manufacturer

Instrument

Throughput

Web site

Abbott ABI

Beckman Coulter

Beckton Dickinson Biomerieux

Bio-Rad Laboratories ml 000 48/run

ABI Prism 6100 Nucleic acid 96/run

Prep Station

ABI Prism 6700 automated 192/run

Nucleic acid workstation

ABI Prism 7000 Detection 96/run System

ABI Prism 7300 Detection 96/run System

ABI Prism 7500 Detection 96/run System

ABI Prism 7900 HT 384/run

Detection System Biomek FX Laboratory Automation Workstation Biomek NX Laboratory Automation Workstation Biomek 2000 Laboratory Automation Workstation Biomek 3000 Laboratory Automation System

BD Viper Sample Processor 550/8 h

Nucleisense Extractor 10/run

EasyMAG 24/run

NucliSens miniMAG 12/run

Easy Q 48/run i Cycler iQ 96/run iCycler iQ5 96/run

MiniOpticon 48/run

Opticon 2 96/run

Chromo 4 96/run

www.abbottdiagnostics.com www.appliedbiosystems.com

www. beckman. com

  • a href="http://www.bd.com">www.bd.com www.biomerieux.com
  • a href="http://www.bio-rad.com">www.bio-rad.com

Amplification General/liquid Amplification/ Extraction preparation_handing_detection x x

XX X

XX X

XX X

XX X

Cephid

Corbett Research

Gen-Probe

Hamilton

Qiagen

Roche

Stratagene Tecan

Smart Cycler 1600 16/run

Smart Cycler 3200 32/run

Smart Cycler 4800 48/run

Smart Cycler 6400 64/run

Smart Cycler 8000 80/run

Smart Cycler 9600 96/run

GeneXpert 1 /cartridge x-tract or gene 96/run

CAS-1200 liquid handing 96/run system

Gradient Palm-Cycler 96-384/run

Rotor-Gene 3000 36-72/run

Tigirs DTS 1000/12 h

MICROLAB STAR^1"

Biorobot EZ1 workstation 6/run

Biorobot M48 workstation 48/run

Biorobot M96 workstation 96/run

Biorobot MDx workstation 96/run

Biorobot 9604 workstation 192/run

MagNA Lyser Instrument 16/run

MagNA Pure Compact System 8/run

MagNA Pure LC System 32/run

Light Cycler 32/run

Cobas Ampliprep 72/run

Cobas TaqMan 48 48/run

Mx3000P 96/run

Mx4000 96/run

Freedom EVO clinical

Ui O

  • a href="http://www.cepheid.com">www.cepheid.com
  • a href="http://www.corbettresearch.com">www.corbettresearch.com
  • a href="http://www.gen-probe.com">www.gen-probe.com
  • a href="http://www.hamiltonrobotics.com">www.hamiltonrobotics.com
  • a href="http://www.qiagen.com">www.qiagen.com
  • a href="http://www.roche-applied-science.com">www.roche-applied-science.com www.roche-diagnostics.com

www. mx3 00Op. co m www.mx4000.com

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