A B C D E F

Figure 8-17 Although antimicrobial susceptibility profiles indicated that several methicillin-resistant S. aureus isolates were he same strain, restriction fragment length polymorphism analysis using pulsed-field gel electrophoresis (Lanes A through F) demonstrates that only isolates B and C were the same.

Figure 8-17 Although antimicrobial susceptibility profiles indicated that several methicillin-resistant S. aureus isolates were he same strain, restriction fragment length polymorphism analysis using pulsed-field gel electrophoresis (Lanes A through F) demonstrates that only isolates B and C were the same.

B C Figure 8-18 Restriction patterns a generated by pulsed-field gel electrophoresis for two Streptococcus pneumoniae isolates, one that was susceptible to penicillin (Lane B) and one that was resistant (Lane C), from the same patient. Restriction fragment length polymorphism analysis indicates that the patient was infected with different strains. Molecular-size markers are shown in Lane A.

Regardless of the method, the process by which enzyme digestion patterns are analyzed is referred to as restriction enzyme analysis (REA). The patterns obtained after gel electrophoresis are referred to as restriction patterns, and differences between microorganism restriction patterns are known as restriction fragment length polymorphisms (RFLPs). Because RFLPs reflect differences or similarities in nucleotide sequences, REA methods can be used for organism identification and for establishing strain relatedness within the same species (see Figures 8-16 to 8-18).

Was this article helpful?

0 0
Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

Get My Free Ebook


Post a comment