Approach To Identification

Complete identification of anaerobes can be costly, often requiring various biochemical tests, gas-liquid chromatography to analyze the metabolic end products of glucose fermentation, and/or gas chromatography for whole-cell long chain fatty acid methyl ester (FAME) analysis. Most clinical laboratories no longer perform complete identification of anaerobes, because presump tive identification is just as useful in assisting the physician in determining appropriate therapy. There^ fore, the approach to identification taken in this chapter emphasizes simple, rapid methods to identify commonly isolated anaerobic bacteria. Identification should proceed in a stepwise fashion, beginning with examination of the primary plates.

examination of primary plates

Anaerobes are usually present in mixed culture v % other anaerobes and facultative bacteria. The combij nation of selective and differential agar plates yield| information that suggests the presence and perhaps tb| types of anaerobe(s). Primary anaerobic plates should t

Bacteroides Fragilis Culture

Figure 44-2 Bacteroides fragilis on anaerobic blood agar.

Bacteroides fragilis on Bacteroides bile esculin agar (Courtesy Anaerobe Systems, Morgan Hill, Calif.)

44-5 Clostridium difficile on cycloserine cefoxitin fructose Sp (CCPA). (Courtesy Anaerobe Systems, Morgan Hill, Calif.)

Agar Pitting Ureolyticus

Figure 44-4 Bacteroides ureolyticus on anaerobic blood agar. Note pitting of agar (arrow). (Courtesy Anaerobe Systems, Morgan Hill, Calif.)

Bacteroides Ureolyticus Gram Stain

Figure 44-6 Gram stain of Clostridium pafringens.

44-5 Clostridium difficile on cycloserine cefoxitin fructose Sp (CCPA). (Courtesy Anaerobe Systems, Morgan Hill, Calif.)

Figure 44-4 Bacteroides ureolyticus on anaerobic blood agar. Note pitting of agar (arrow). (Courtesy Anaerobe Systems, Morgan Hill, Calif.)

Figure 44-6 Gram stain of Clostridium pafringens.

Figure 44-2 Bacteroides fragilis on anaerobic blood agar.

Bacteroides fragilis on Bacteroides bile esculin agar (Courtesy Anaerobe Systems, Morgan Hill, Calif.)

Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

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