B

Specimen is placed

Specimen is placed

Figure 5-4 Methods of inoculating solid media. A, Streaking for quantitation. B, Streaking semiquantitatively.

loop is then flipped over or flamed and quadrant two is struck by pulling the loop through quadrant one a few times and streaking the rest of the area. The nichrome loop is then flamed again and quadrant three is streaked by going into quadrant two a few times and streaking the rest of the area, finally quadrant four is streaked by pulling the loop over the rest of the agar without further flaming. This process is called streaking for isolation, because the microorganisms present in the specimen are successively diluted out as each quadrant is streaked until finally each morphotype is present as a single colony. Numbers of organisms present can subsequently be graded as 4+ (many, heavy growth) if growth is out to the fourth quadrant, 3+ (moderate growth) if growth is out to the third quadrant, 2+ (few or light growth) if growth is in the second quadrant, and 1+ (rare) if growth is in the first quadrant. This tells the clinician the relative numbers of different organisms present in the specimen; such semiquantitative information is usually sufficient for the physician to be able to treat the patient.

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Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

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