Leukocytes, which contain Fc receptors for antibody, can also cause nonspecific binding of antibody conjugates. To verify one's ability to interpret FA tests, every laboratory should perform viral culture or some alternative detection method in parallel with immunofluorescence until in-house performance has been established.
Immunofluorescent stains that are most useful i the clinical virology laboratory are those for RSV, influenza and parainfluenza viruses, adenovirus, HS VZV, and CMV. A pool of antibodies can be used t« screen a specimen for multiple viruses. A positive screeD is then tested with each individual reagent to identify the exact virus that is present. Screening pools have been used successfully for the detection of respiratory viruses
Figure 51-15 Electron micrographs of viruses. A, Rotavirus. B, Adenovirus.
C, Norwalk agent virus.
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