Nonspecific agglutination should not occur.
Culture. Abscess and biopsy specimens submitted for culture should be inoculated onto 5% sheep blood and chocolate agar plates. Plates should be incubated m 5% to 10% C02 for 72 hours at 35° C. In addition, an anaerobic agar plate and broth with an anaerobic indicator, vitamin K, and hemin should be inoculated and incubated in an anaerobic environment at 35° C. Anaerobic culture plates are incubated for a minimum of 72 hours but are examined after 48 hours of incubation. Anaerobic broths should be incubated for a minimum of 5 days. If a fungal etiology is suspected, fungal media such as brain-heart infusion with blood and antibiotics or inhibitory mold agar, should be inoculated as well.
A 2-year-old girl presented at midnight to the hospital emergency department with a temperature of 104° F. She was diagnosed with bilateral otitis. Her parents were given a prescription for amoxicillin/ davulanic add, which they were unable to have filled that night. In the morning they found the child lethargic and having seizures. They returned to the hospital where the child presented with fever of 105° C, purpura, and nuchal rigidity. CSF cultures were collected. The child was started on ceftriaxone. Hie next day the laboratory reported growth of a gramnegative diplococcus.
Was this article helpful?