Incubation Conditions

Inoculated media are incubated under various temperatures and environmental conditions, depending on the

First quadrant

Fourth quadrant

Third quadrant

Third quadrant

Specimen is swabbed or dropped by pipette on a small area of the plate

First quadrant

Fourth quadrant organisms being sought, for example, 28° C for fungi and 35° to 37° C for most bacteria, viruses, and acid-fast bacillus. A number of different environmental conditions exist. Aerobes grow in ambient air, which contains 21% oxygen (02) and a small amount (0.03%) of carbon dioxide (C02). Anaerobes usually cannot grow in the presence of 02 and the atmosphere in anaerobe jars, bags, or chambers is composed of 5% to 10% hydrogen (H2), 5% to 10% C02, 80% to 90% nitrogen (N2), and 0% 02. Capnophiles, such as Haemophilus influenzae and Neisseria gonorrhoeae, require increased concentrations of C02 (5% to 10%) and approximately 15% 02. This atmosphere can be achieved by a candle jar (3% C02) or a C02 incubator, jar, or bag. Microaerophiles (Campylobacter jejuni, Helicobacter pylori) grow under reduced 02 (5% to 10%) and increased C02 (8% to 10%). This environment can also be obtained in specially designed jars or bags.

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Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

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