Inoculated media are incubated under various temperatures and environmental conditions, depending on the
Specimen is swabbed or dropped by pipette on a small area of the plate
Fourth quadrant organisms being sought, for example, 28° C for fungi and 35° to 37° C for most bacteria, viruses, and acid-fast bacillus. A number of different environmental conditions exist. Aerobes grow in ambient air, which contains 21% oxygen (02) and a small amount (0.03%) of carbon dioxide (C02). Anaerobes usually cannot grow in the presence of 02 and the atmosphere in anaerobe jars, bags, or chambers is composed of 5% to 10% hydrogen (H2), 5% to 10% C02, 80% to 90% nitrogen (N2), and 0% 02. Capnophiles, such as Haemophilus influenzae and Neisseria gonorrhoeae, require increased concentrations of C02 (5% to 10%) and approximately 15% 02. This atmosphere can be achieved by a candle jar (3% C02) or a C02 incubator, jar, or bag. Microaerophiles (Campylobacter jejuni, Helicobacter pylori) grow under reduced 02 (5% to 10%) and increased C02 (8% to 10%). This environment can also be obtained in specially designed jars or bags.
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