Löwenstein-Jensen (L-J) L-J with RNA L-J with pyruvic acid Middlebrook 7H10

If growth occurs, confirm by AFB smear

Confirm by AFB smear

Reinocúlate solid media

^Mycobacterium Growth Indicator Tube.

Figure 45-1 A flowchart for specimen processing for isolation of mycobacteria.

Digestion-decontamination procedures should be as gentle as possible, with no more than an overall contamination rate of 5%.5

Overview. Commonly used digestion^eœntamina-tion methods are the sodium hydroxide (NaOH) method the Zephiran-trisodium phosphate method, and the JV-acetyl-l-cysteine (NALC)-NaOH method. The NALC-NaOH method is detailed in Procedure 45-1. Of note, another decontaminating procedure using oxalic acid is very useful for treating specimens known to harbor gram-negative rods, particularly Pseudomonas and Proteus, which are extremely troublesome conta minants. NaOH is a commonly used decontaminant th< is also mucolytic. Several agents can be used to liquefy^ clinical specimen, including NALC, dithiothreitol (spl tolysin), and enzymes. None of these agents are inbH bitory to bacterial cells. In most procedures, liquefacd^j (release of the organisms from mucin or cells) is enhanced by vigorous mixing with a vortex type of mixei, in a closed container. Following mixing, the contain^ should be allowed to stand for 15 minutes before ope#jj ing, to prevent the dispersion of fine aerosols generate during mixing. Of utmost importance during process™ is strict adherence to processing and laboratory safera protocols. All of these procedures should be carried

1v-Acetvl-l-Cysteine-Sodium Hydroxide Method for Liquefaction and Decontamination of Specimens

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Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

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