J

C Separation process to remove unbound, labeled probes

D Purified duplexes read for detection of reporter molecule

Purified duplexes . ready for detection f reporter molecule

Figure 8-3 Principle of the solution hybridization format.

to "flood" the membrane and allow for hybridization to occur. After a series of incubations and washings to remove unbound probe, the membrane is processed for detection of duplexes (Figure 8-4, A). An advantage of this method is that a single membrane can hold several samples for exposure to the same probe.

Southern hybridization is another method that uses membranes as the solid support, but in this instance the nudeic add target has been purified from the organisms and digested with specific enzymes to produce several fragments of various sizes (see Figure 8-4, B; also see the section tided, "Enzymatic Digestion and Electrophoresis of Nuddc Adds" later in this chapter). By gel electrophoresis, the nuddc add fragments, which carry a net negative charge, are

Figure 8-4 Principle of solid support hybridization formats. A, Filter hybridization. B, Southern hybridization. C, Sandwich hybridization.

A Fitter hybridization

Target nucleic acid released and denatured to single strand

Single-stranded target affixed to membrane i

Addition of labeled probe

Addition of labeled probe

Hybridization

Wash and ^ detection of " reporter molecule

Sample affixed to membrane

Single-stranded target affixed to membrane

Hybridization

I Southern hybridization

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Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

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