Specimen Collection and Transport
See General Considerations for the Laboratory Diagnosis: of Fungal Infections.
See General Considerations for the Laboratory Diagno^ of Fungal Infections.
Direct Detection Methods
Stains. The microscopic morphologic features ® the tissue forms, or what has been termed the parasit^
forms, of the dimorphic fungi vary with the genus and are described for each below.
Figure 50-69 The tissue form of Coccidioides immitis (i.e„ the spherule). The external wall of the spherule does not stain with the silver stain, whereas the internal endospores do stain (arrowhead). Also note how the juxtaposed endospores, which have been released from a spherule that has burst, resemble a budding yeast (arrow). GMS stain (400x).
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