Methods

  1. Viruses other than CM V and VZV a. Select tubes showing a2+ CPE or hemadsorption. RBCs may be eluted following hemadsorption by placing tubes in a 37° C water bath for 15 minutes. Wash to remove RBCs. In all steps, open cell culture tubes in biological safety cabinet.
  2. Add three sterile glass beads to cell culture tube. Tighten cap. Vortex vigorously behind shield for 30 seconds. Examine microscopically to ensure that cells have been disrupted and removed from glass surface.
  3. Add cells and medium to freezer tube. Snap freeze by swirling the tube in a slurry of dry ice and acetone, d. Store at-70° C.
  4. CMV and VZV: Use tubes showing a3+ CPE. Harvest as above except add 10% DMSO and glass beads. For example, add 0.2 mL of DMSO to 2 mL of medium in cell culture tube. Mix and proceed as described above.
  5. Thaw in a 37° C water bath. Inoculate to appropriate cell culture.

A previously healthy 19-year-old college student presented to the hospital emergency department with symptoms of respiratory tract infection. The patient experienced severe shortness of breath, was transferred to the intensive care unit, and was intubated and placed on a ventilator. A chest radiograph revealed left lung infiltrates.

questions

  1. What viruses cause serious respiratory tract disease in young adults?
  2. What specimens are recommended to help diagnose viral respiratory tract infection?
  3. What viral diagnostic tests would be appropriate for the detection of respiratory tract viruses?

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Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

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