Other Immunoassays

Several other methods, including radioimmunoassay (RIA) and fluorescent immunoassay (FIA), are similar to ELISA except that radionudeotides (usually 12SI or ,4C) are substituted for enzymes in RIA and fluoro-chromes are substituted for enzymes in FIA. Although RIA was formerly the key method for antigen detection for numerous infectious agents, including hepatitis B virus, it has been largely replaced by ELISA testing, which does not require use of radioactive substances.

An optical immunoassay (OIA) has recently been introduced to detect group A streptococci pharyngitis (Figure 9-16). It detects changes in the reflection of light occurring on the inert matrix after antigen and antibody have attached to it.

Antigen

Was this article helpful?

0 0
Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

Get My Free Ebook


Post a comment