Several serologic methods for detecting antibodies to Bartonella spp. have been developed. An indirect fluorescent antibody using antigen prepared from Bartonella spp. co-cultivated with Vero cells18 and enzyme-linked_I
immunoassays has been developed and evaluates® However, the sensitivity and specificity of these assays have been questioned. Cross-reactivity between Bartonella and Chlamydia spp. and Coxiella burnettii have
reported.17 Of note, a significant antibody response to infection is not mounted in -most HIV-infected patients, who as a group are susceptible to Bartonella infections.10
In conclusion, LaScola and colleagues in a 5-year atuc y of various samples obtained for culture of Bartonella species demonstrated that the success of recovery iff detection of B. henselae or B. quintana was dependent on several factors. These factors include the clinical form of he disease (i.e., endocarditis, bacteremia, bacillary angiomatosis, or CSD), previous antibiotic therapy, the type of clinical specimen (e.g., blood, heart valve, skin, or lymph node), and the type of laboratory diagnostic method employed (serology, PCR, shell vial cultures " h human endothelial cell monolayers, direct plating K blood onto agar or broth blood cultures).13
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