Specimen Processing Direct Visual Examination

Lower respiratory tract specimens can be examined by direct wet preparation for some parasites and by special procedures for Pneumocystis, Fungal elements can be visualized under phase microscopy with 10% potassium hydroxide, under ultraviolet light with calcofluor white, or using periodic acid-Schiff-stained smears.

For most other evaluations, the specimen must be fixed and stained. Bacteria and yeasts can be recognized on Gram stain. One of the most important uses of the Gram stain, however; is to evaluate the quality of ex-

Respiratory Specimens With ParasiteBronchial Cannula

Outer cannula Inner cannula

Gram Stain

Brush

Purulent exudate

Respiratory Specimens With Parasite

Outer cannula Inner cannula

Brush

Purulent exudate

Figure 53-3 Overview of obtaining a protected catheter bronchial brush during a bronchoscopy examination. A, The bronchoscope is inToduced into the nose and advanced through the nasopharyngeal passage into the trachea. The bronchoscope then mserted nUo hetoga"a B, A small brush that Lids 0.0 Ho O.MlmL of secretions is placed within a doublera. The end rf omermosTtube or cannula is closed with a displaceable plug made of absorbable gel. The cannula is insertedI to^he proper area COnce inthe correct area, the inner cannula is pushed out, dislodging the protective plug as it is extruded. D The brush is then extended beyond the innei cannula, and the specimen is collected by Pushing'' theinvolved area. The bn^shis the inner cannula, which is withdrawn into the outer cannula to prevent contamination by upper airway organisms as it .s removed.

Was this article helpful?

0 0
Bacterial Vaginosis Facts

Bacterial Vaginosis Facts

This fact sheet is designed to provide you with information on Bacterial Vaginosis. Bacterial vaginosis is an abnormal vaginal condition that is characterized by vaginal discharge and results from an overgrowth of atypical bacteria in the vagina.

Get My Free Ebook


Post a comment